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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-14-3-3 eta antibody
See all 14-3-3 eta products (5) ...
Description
Rabbit polyclonal to 14-3-3 eta
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide, corresponding to a region between amino acids 71-120 of Human 14-3-3 eta
Positive control
WB: human fetal brain IHC: human skin and intestine
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels.
Sequence similarities
Belongs to the 14-3-3 family.
Target information above from: UniProt accessionQ04917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 14 3 3 protein eta antibody
- 14-3-3 protein eta antibody
- 1433F_HUMAN antibody
see all
Database links
- Entrez Gene: 282126 Cow
- Entrez Gene: 7533 Human
- Entrez Gene: 22629 Mouse
- Entrez Gene: 25576 Rat
- Omim: 113508 Human
- SwissProt: P68509 Cow
- SwissProt: Q04917 Human
- SwissProt: P68510 Mouse
see all
Anti-14-3-3 eta antibody images:
Western blot - 14-3-3 eta antibody (ab28665)
Anti-14-3-3 eta antibody (ab28665) at 0.12 µg/ml + Human fetal brain lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
developed using the ECL technique
Predicted band size : 28 kDa
Observed band size : 28 kDa
Immunohistochemistry (Paraffin-embedded sections) - 14-3-3 eta antibody (ab28665)
14-3-3 eta expression detected in paraffin-embedded sections of human skin, using ab28665 at 4-8ug/ml. The arrows indicate positive staining of squamous epithelial cells.
Immunohistochemistry (Paraffin-embedded sections) - 14-3-3 eta antibody (ab28665)
14-3-3 eta expression detected in paraffin-embedded sections of human intestine, using ab28665 at 4-8ug/ml. The arrows indicate positive staining of epithelial cells of intestinal villi.
References for Anti-14-3-3 eta antibody (ab28665)
This product has been referenced in:
- Morris Ket al. Cofilin and slingshot localization in the epithelium of uterine endometrium changes during the menstrual cycle and in endometriosis. Reprod Sci 18:1014-24 (2011).Read more (PubMed: 21693774) »
- Moreira JMet al. A combined proteome and ultrastructural localization analysis of 14-3-3 proteins in transformed human amnion (AMA) cells: definition of a framework to study isoform-specific differences. Mol Cell Proteomics 7:1225-40 (2008).Read more (PubMed: 18378962) »
See all 2 publications for this product
Publishing research using ab28665? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"