|
|
|
|
Western blot - 14-3-3 eta antibody (ab58073)

(enlarge)
|
|
|
|
|
Product Name
|
14-3-3 eta antibody
|
| |
See all 14-3-3 eta antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal to 14-3-3 eta
|
|
Immunogen
|
Recombinant fragment: MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP , corresponding to amino acids 71-170 of Human 14-3-3 eta BLAST 'MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP ' with or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3 Signal Transduction >> Adapters >> Cytoplasmic
|
|
Relevance
|
14-3-3 eta (YWHAH) belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine or phosphothreonine-containing proteins. YWHAH is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to a large number of partners and binding generally results in the modulation of the activity of the binding partner. The YWHAH gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Isotype
|
IgG2a
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: None PBS, pH 7.2
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 14-3-3 eta antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
14-3-3 eta antibody images:
|
Western blot - 14-3-3 eta antibody (ab58073)
Western blot against tagged recombinant protein immunogen using ab58073 14-3-3 eta antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to 14-3-3 eta
|
| |
14-3-3 eta antibody - more information
|
Customer reviews (feedback) regarding 14-3-3 eta antibody |
Customer FAQs regarding 14-3-3 eta antibody |
Protocols for 14-3-3 eta antibody |
Price and availability of products related to 14-3-3 eta antibody |
| |
14-3-3 eta antibody - other tools:
|
Contact Abcam with a Technical Enquiry about 14-3-3 eta antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"