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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-14-3-3 eta antibody
See all 14-3-3 eta products (5) ...
Description
Rabbit polyclonal to 14-3-3 eta
Specificity
Detects endogenous levels of total 14-3-3 eta protein.
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide (Human) from an internal region
Positive control
Jurkat cell extract HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 40 kDa (predicted molecular weight: 28 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels.
Sequence similarities
Belongs to the 14-3-3 family.
Target information above from: UniProt accessionQ04917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 14 3 3 protein eta antibody
- 14-3-3 protein eta antibody
- 1433F_HUMAN antibody
see all
Database links
- Entrez Gene: 7533 Human
- Entrez Gene: 22629 Mouse
- Entrez Gene: 25576 Rat
- Omim: 113508 Human
- SwissProt: Q04917 Human
- SwissProt: P68510 Mouse
- SwissProt: P68511 Rat
- Unigene: 226755 Human
see all
Anti-14-3-3 eta antibody images:
Western blot - 14-3-3 eta antibody (ab69591)
All lanes : Anti-14-3-3 eta antibody (ab69591) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 28 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - 14-3-3 eta antibody (ab69591)
Immunofluorescence analysis of HeLa cells, using ab69591 at 1/500-1/1000 dilution.
References for Anti-14-3-3 eta antibody (ab69591)
ab69591 has not yet been referenced specifically in any publications.
Publishing research using ab69591? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"