Anti-14-3-3 eta + gamma + zeta antibody (ab14124)

Overview

  • Product nameAnti-14-3-3 eta + gamma + zeta antibody
  • Description
    Rabbit polyclonal to 14-3-3 eta + gamma + zeta
  • SpecificityRecognizes zeta, gamma and eta isoforms at 27kDa in various mammalian systems.
  • Tested applicationsSuitable for: ELISA, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Cow
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide:

    KMKGDYYRYLAE

    , corresponding to amino acids 124-135 of 14-3-3 eta + gamma + zeta.

  • Positive control
    • Bovine brain cytosol.
  • General notes


    14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and hetero-dimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. At least seven isoforms of 14-3-3 have been identified with the zeta isoform being the most broadly expressed in animals.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: Whole Serum
  • PurityWhole antiserum
  • Primary antibody notes14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and hetero-dimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. At least seven isoforms of 14-3-3 have been identified with the zeta isoform being the most broadly expressed in animals.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/10000. Predicted molecular weight: 27 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • Cellular localizationCytoplasmic
    • Alternative names
      • 14 3 3 eta antibody
      • 14 3 3 gamma antibody
      • 14 3 3 protein eta antibody
      • 14 3 3 protein gamma antibody
      • 14 3 3 protein zeta antibody
      • 14 3 3 protein zeta/delta antibody
      • 14 3 3 protein/cytosolic phospholipase A2 antibody
      • 14 3 3 zeta antibody
      • KCIP 1 antibody
      • KCIP1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • Phospholipase A2 antibody
      • Protein AS 1 antibody
      • Protein AS1 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide antibody
      • YWHA 1 antibody
      • YWHA1 antibody
      • YWHAG antibody
      • Ywhah antibody
      • YWHAZ antibody
      see all

    References for Anti-14-3-3 eta + gamma + zeta antibody (ab14124)

    ab14124 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"