![Western blot - 14-3-3 gamma antibody [3F8] (ab14118) image](#ab14118_1.jpg)
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Product name
Anti-14-3-3 gamma antibody [3F8]
See all 14-3-3 gamma products (9) ...
Description
Mouse monoclonal [3F8] to 14-3-3 gamma
Specificity
No crossreactivity with other isoforms of 14-3-3.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human 14-3-3 gamma).
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
3F8
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - 14-3-3 gamma antibody [3F8] (ab14118)
![Western blot - 14-3-3 gamma antibody [3F8] (ab14118) image](/ps/datasheet/Images/14/ab14118/ab14118_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart.
Sequence similarities
Belongs to the 14-3-3 family.
Post-translational
modifications
Phosphorylated by various PKC isozymes.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP61981
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- N-terminally processed antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Database links
- Entrez Gene: 7532 Human
- Omim: 605356 Human
- SwissProt: P61981 Human
- Unigene: 721582 Human
Anti-14-3-3 gamma antibody [3F8] images:
Western blot - 14-3-3 gamma antibody [3F8] (ab14118)
All lanes : Anti-14-3-3 gamma antibody [3F8] (ab14118) at 1/2000 dilution
Lane 1 : 5ug U251 human glioma whole cell lysate
Lane 2 : 5ug CFBE human airway epithelium
Lane 3 : 5ug COS7 lysate
Lane 4 : 5ug Calu3 human airway epithelium
Secondary
HRP conjugated Goat anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 second
This image is courtesy of an Abreview submitted by Ms Baby Ribbon
References for Anti-14-3-3 gamma antibody [3F8] (ab14118)
This product has been referenced in:
- Pereira-Faca SR et al. Identification of 14-3-3 theta as an antigen that induces a humoral response in lung cancer. Cancer Res 67:12000-6 (2007).Read more (PubMed: 18089831) »
See 1 publication for this product
Publishing research using ab14118? Please let us know so that we can cite the reference in this datasheet
![14-3-3 gamma antibody [3F8] for WB in Human (14118)](/ps/Reviews/Images/ab5648_19617.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"