![Western blot - 14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525) image](#1431a.jpg)
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Product name
Anti-14-3-3 gamma antibody [CG31] - BSA and Azide free
See all 14-3-3 gamma products (9) ...
Description
Mouse monoclonal [CG31] to 14-3-3 gamma - BSA and Azide free
Specificity
This antibody is highly specific to 14-3-3 gamma and shows no cross reaction with other isoforms of 14-3-3.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length human 14-3-3 gamma protein
Positive control
HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from ascites fluid by Protein G chromatography.
Clonality
Monoclonal
Clone number
CG31
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - 14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525)
![Western blot - 14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525) image](/ps/datasheet/Images/76/ab76525/1431a.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab76525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: Use at 2 µg/mg of lysate. Denatured verified. Use Protein G.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart.
Sequence similarities
Belongs to the 14-3-3 family.
Post-translational
modifications
Phosphorylated by various PKC isozymes.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP61981
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- N-terminally processed antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Database links
- Entrez Gene: 7532 Human
- Omim: 605356 Human
- SwissProt: P61981 Human
- Unigene: 721582 Human
Anti-14-3-3 gamma antibody [CG31] - BSA and Azide free images:
Western blot - 14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525)
Anti-14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525) at 2 µg/ml + Human cells 8
Predicted band size : 30 kDa
Observed band size : 30 kDa
Note: a different version of ab76525, a 14-3-3 gamma antibody containing BSA and sodium azide, was used to obtain this image.
References for Anti-14-3-3 gamma antibody [CG31] - BSA and Azide free (ab76525)
ab76525 has not yet been referenced specifically in any publications.
Publishing research using ab76525? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"