Recombinant
RabMAb

Anti-14-3-3 gamma antibody [EPR9655(B)] (ab137106)

Overview

  • Product name
    Anti-14-3-3 gamma antibody [EPR9655(B)]
    See all 14-3-3 gamma primary antibodies
  • Description
    Rabbit monoclonal [EPR9655(B)] to 14-3-3 gamma
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, A431, 293T and K562 cell lysates; K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab137106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • Tissue specificity
      Highly expressed in brain, skeletal muscle, and heart.
    • Sequence similarities
      Belongs to the 14-3-3 family.
    • Post-translational
      modifications
      Phosphorylated by various PKC isozymes.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 3 3 gamma antibody
      • 14 3 3 protein gamma antibody
      • 14 3 3 protein gamma subtype antibody
      • 14 3 3gamma antibody
      • 14-3-3 protein gamma antibody
      • 1433G_HUMAN antibody
      • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
      • KCIP 1 antibody
      • KCIP-1 antibody
      • KCIP1 antibody
      • N-terminally processed antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
      • Ywhag antibody
      see all

    Images

    • All lanes : Anti-14-3-3 gamma antibody [EPR9655(B)] (ab137106) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
    • Flow cytometric analysis of K562 cells labelling 14-3-3-gamma with ab137106 at 1/10 dilution (red). Negative Rabbit IgG control shown in green.

    References

    ab137106 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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