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Product name
Anti-14-3-3 gamma antibody - N-terminal
See all 14-3-3 gamma products (9) ...
Description
Rabbit polyclonal to 14-3-3 gamma - N-terminal
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide selected from the N-terminal region of Human 14-3-3 gamma conjugated to KLH (NP_036611).
Positive control
A375 cell line lysates and Mouse cerebellum tissue lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab91547 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab91547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
ELISA
Application notes
ELISA: 1/1000.
WB: 1/100 - 1/500. Predicted molecular weight: 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart.
Sequence similarities
Belongs to the 14-3-3 family.
Post-translational
modifications
Phosphorylated by various PKC isozymes.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP61981
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- N-terminally processed antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Database links
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Omim: 605356 Human
- SwissProt: P61981 Human
- SwissProt: P61982 Mouse
- Unigene: 721582 Human
- Unigene: 233813 Mouse
- Unigene: 482082 Mouse
Anti-14-3-3 gamma antibody - N-terminal images:
Western blot - 14-3-3 gamma antibody - N-terminal (ab91547)
Anti-14-3-3 gamma antibody - N-terminal (ab91547) at 1/100 dilution + A375 cell line lysates at 35 µg
Predicted band size : 28 kDa
Western blot - 14-3-3 gamma antibody - N-terminal (ab91547)
Anti-14-3-3 gamma antibody - N-terminal (ab91547) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg
Predicted band size : 28 kDa
References for Anti-14-3-3 gamma antibody - N-terminal (ab91547)
ab91547 has not yet been referenced specifically in any publications.
Publishing research using ab91547? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"