This antibody gave a positive signal in the following lysates: Human brain tissue; Human skeletal muscle tissue; Mouse brain tissue; HeLa Whole Cell; NIH 3T3 Whole Cell; K562 Whole Cell. ICC/IF: SKNSH cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Fix cells using paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificityHighly expressed in brain, skeletal muscle, and heart.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsPhosphorylated by various PKC isozymes.
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Anti-14-3-3 gamma antibody images
Western blot - Anti-14-3-3 gamma antibody (ab102673)
All lanes : Anti-14-3-3 gamma antibody (ab102673) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 28 kDa Observed band size : 28 kDa Additional bands at : 150 kDa,40 kDa,60 kDa,90 kDa,>250 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma antibody (ab102673)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab102673 (1/200) staining 14-3-3 gamma in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
ICC/IF image of ab102673 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102673, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-14-3-3 gamma antibody (ab102673)
has not yet been referenced specifically in any publications.
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