Anti-14-3-3 gamma antibody (ab155050)

Overview

  • Product name
    Anti-14-3-3 gamma antibody
    See all 14-3-3 gamma primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 gamma
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-179 of Human 14-3-3 gamma (UniProt ID: P61981).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysate; HeLa cells; Human DLD1 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab155050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Anti-14-3-3 gamma antibody images

  • All lanes : Anti-14-3-3 gamma antibody (ab155050) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
  • Anti-14-3-3 gamma antibody (ab155050) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 28 kDa
  • Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma with ab155050 at 1/500 dilution.

References for Anti-14-3-3 gamma antibody (ab155050)

ab155050 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Bos taurus Cell lysate - whole cell (BoMAC (SV40-transformed bovine macrophages) and HE)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
BoMAC (SV40-transformed bovine macrophages) and HE
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Mar 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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