• Product name
    Anti-14-3-3 gamma antibody
    See all 14-3-3 gamma primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 gamma
  • Host species
  • Specificity
    This antibody detects endogenous levels of total 14-3-3 gamma protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal of human 14-3-3 gamma.

  • Positive control
    • Human brain tissue K562 cell extract treated with insulin Cos7 cells



Our Abpromise guarantee covers the use of ab69592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.


  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity
    Highly expressed in brain, skeletal muscle, and heart.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    Phosphorylated by various PKC isozymes.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 gamma antibody
    • 14 3 3 protein gamma antibody
    • 14 3 3 protein gamma subtype antibody
    • 14 3 3gamma antibody
    • 14-3-3 protein gamma antibody
    • 1433G_HUMAN antibody
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
    • Ywhag antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab69592 at 1/50-1/100 dilution. Right hand panel was treated with immunising peptide.
  • All lanes : Anti-14-3-3 gamma antibody (ab69592) at 1/500 dilution

    Lane 1 : K562 cell extract treated with insulin (0.01U/ml, 15mins)
    Lane 2 : K562 cell extract treated with insulin (0.01U/ml, 15mins) with peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of COS7 cells, using ab69592 at 1/500-1/1000 dilution. Right hand panel was treated with immunising peptide.


This product has been referenced in:
  • Lee YS  et al. Suppression of 14-3-3?-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells. Sci Rep 6:26413 (2016). Read more (PubMed: 27212225) »
  • Broadbelt KG  et al. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics 11:M111.009530 (2012). WB, ICC/IF ; Human . Read more (PubMed: 21976671) »

See all 2 Publications for this product

Customer reviews and Q&As

Many thanks for the reply.

I am pleased to hear that the staining now seems to have improved for you and the problems initially encountered have been resolved. This information is very helpful to us in monitoring the quality of our products. Read More

Thank you for contacting us yesterday to chase up your enquiry into the Anti-14-3-3 gamma antibody (ab69592). I am sorry that your original enquiry was not dealt with as it should have been. We aim to reply to all our customers within a 24 hour period ...

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Thank you for contacting us.

I have heard back for ab63635, ab69592:

Unfortunately, for these 2 antibodies the immunogen is not available for sale.

As for ab97273 and ab43057, I am still waiting for a reply and will let y...

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Western blot
Mouse Tissue lysate - whole (brain)
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Dec 15 2010


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