Anti-14-3-3 gamma antibody [J3H10] (ab118877)

Overview

  • Product nameAnti-14-3-3 gamma antibody [J3H10]
    See all 14-3-3 gamma primary antibodies
  • Description
    Mouse monoclonal [J3H10] to 14-3-3 gamma
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-274 of Human 14-3-3 gamma (NP_036611) purified from E. coli.

  • Positive control
    • Human brain cortex or heart tissue. HeLa cell lysate; mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificityHighly expressed in brain, skeletal muscle, and heart.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    Phosphorylated by various PKC isozymes.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 gamma antibody
    • 14 3 3 protein gamma antibody
    • 14 3 3 protein gamma subtype antibody
    • 14 3 3gamma antibody
    • 14-3-3 protein gamma antibody
    • 1433G_HUMAN antibody
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
    • Ywhag antibody
    see all

Anti-14-3-3 gamma antibody [J3H10] images

  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab118877at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Heart (formalin-fixed, paraffin-embedded) stained with ab118877 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-14-3-3 gamma antibody [J3H10] (ab118877) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 28 kDa

References for Anti-14-3-3 gamma antibody [J3H10] (ab118877)

ab118877 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118877.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"