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- Secondary antibodies
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Product name
Anti-14-3-3 (phospho S58) antibody
See all 14-3-3 products (10) ...
Description
Rabbit polyclonal to 14-3-3 (phospho S58)
Specificity
Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Zebrafish
Predicted to work with
Sheep, Chicken, Cow, Dog, Xenopus laevis, Non Human Primates
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 29 kDa.
IHC-Fr: 1/500.
Target
Relevance
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.
Cellular localization
Cytoplasmic
Alternative names
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
Database links
- Entrez Gene: 419190 Chicken
- Entrez Gene: 425619 Chicken
- Entrez Gene: 286863 Cow
- Entrez Gene: 287022 Cow
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 22631 Mouse
see all
Anti-14-3-3 (phospho S58) antibody images:
Western blot - 14-3-3 (phospho S58) antibody (ab30554)
All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution
Lane 1 : Rat brain stem lysate
Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Frozen sections) - 14-3-3 (phospho S58) antibody (ab30554)
ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.
Immunocytochemistry/ Immunofluorescence - 14-3-3 (phospho S58) antibody (ab30554)
ab30554 staining 14-3-3 (phospho S58) in human U2OS cells by Immunocytochemistry/ Immunofluorescence.U2OS cells were plated on coverslips and fixed by sequential incubations with 4% formaldehyde in PBS without calcium and magnesium [PBS(-)], 0.1% Triton X-100 in PBS(-) and 0.05% Tween-20 in PBS(-), each for 10 minutes at room temperature. After being washed, cells were incubated with ab30554, followed by incubation with Alexa Fluor 488–conjugated anti-rabbit IgG in TBST (100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.05% Tween-20) containing 5% FBS.
Image from Okada N et al, J Cell Sci. 2011 Jan 1;124(Pt 1):57-67. Epub 2010 Nov 30, Fig 6. DOI 10.1242/?jcs.072918
Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)
All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Mouse brain homogenate (20ug)
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
References for Anti-14-3-3 (phospho S58) antibody (ab30554)
This product has been referenced in:
- Okada N et al. A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. J Cell Sci 124:57-67 (2011). WB, ICC/IF; Human.Read more (PubMed: 21118956) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"