Anti-14-3-3 sigma (acetyl10) antibody (ab111305)

Overview

  • Product nameAnti-14-3-3 sigma (acetyl10) antibody
    See all 14-3-3 sigma primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 sigma (acetyl10)
  • SpecificityRecognizes 14-3-3 sigma in all mammals.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Sheep
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Human, all Mammals
  • Immunogen

    Synthetic peptide:

    MERASLIQKAC

    , corresponding to acetylated N terminal amino acids 1-10 of 14-3-3 sigma (O77642).

  • Positive control
    • Human Skin (IHC-P), HEK293 cell lysates (WB)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab111305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. Detects a band of approximately 30kD in HEK293 cell lysates.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma (acetyl10) antibody images

  • Immunohistochemistry Image of Human Skin (formalin fixed, paraffin embedded) stained with ab111305 at 1:250. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

References for Anti-14-3-3 sigma (acetyl10) antibody (ab111305)

ab111305 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"