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Recombinant full length protein (Human).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab151504 as a replacement.
Our Abpromise guarantee covers the use of ab14123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. Use 2µg/mg protein lysate. Use protein G.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. 2 hours at RT.|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab14123 staining mouse embryonic skin tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with a commercially available (mouse-on-mouse) blocking reagent for 1 hour at RT. The primary antibody was diluted 1/50 and incubated with the sample for 24 hours at 4°C. A biotinylated mouse anti-mouse antibody was used as the secondary, followed by a Streptavidin-Cy3®.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"