Anti-14-3-3 sigma antibody [1433S01] (ab14123)

Overview

  • Product name
    Anti-14-3-3 sigma antibody [1433S01]
    See all 14-3-3 sigma primary antibodies
  • Description
    Mouse monoclonal [1433S01] to 14-3-3 sigma
  • Specificity
    Recognizes human 14-3-3 sigma protein, 26-30kD. Does not crossreact with other isoforms of 14-3-3. The clone number has been updated from (1.N.6) to (1433S01) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HT29 cells and skin

Properties

Applications

Our Abpromise guarantee covers the use of ab14123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use 2µg/mg protein lysate. Use protein G.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. 2 hours at RT.
IHC-P Use a concentration of 2 - 4 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity
    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization
    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody [1433S01] images

  • ab14123 staining mouse embryonic skin tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with a commercially available (mouse-on-mouse) blocking reagent for 1 hour at RT.  The primary antibody was diluted 1/50 and incubated with the sample for 24 hours at 4°C.   A biotinylated mouse anti-mouse antibody was used as the secondary, followed by a Streptavidin-Cy3®. 

    See Abreview

  • All lanes : Anti-14-3-3 sigma antibody [1433S01] (ab14123) at 0.5 µg/ml

    Lane 1 : U251 (non malignant glioma cells)
    Lane 2 : CFBE41o- cells stably transfected with wild-type cystic fibrosis transmembrane conductance regulator (CFTR).
    Lane 3 : CFBE41o- cells stably transfected with deltaF508 of cystic fibrosis transmembrane conductance regulator (CFTR).

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti mouse (HRP) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    This image is an edited version of an image received courtesy of an Abreview submitted on 10 October 2005. We do not have any further information relating to this image.

    See Abreview

  • ab14123 at 1/50 staining human epithelium tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the antibody for 30 minutes. An HRP conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab14123 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-14-3-3 sigma antibody [1433S01] (ab14123)

This product has been referenced in:
  • Khanom R  et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016). Read more (PubMed: 27512993) »
  • Seok JK & Boo YC p-Coumaric Acid Attenuates UVB-Induced Release of Stratifin from Keratinocytes and Indirectly Regulates Matrix Metalloproteinase 1 Release from Fibroblasts. Korean J Physiol Pharmacol 19:241-7 (2015). WB ; Human . Read more (PubMed: 25954129) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tongue)
Specification
tongue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1 mM EDTA
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (head and neck cancer)
Specification
head and neck cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Dr. Satyendra Tripathi

Verified customer

Submitted Jul 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
10000 cells
Specification
A549 cells
Treatment
26 nM DMSO/bortezomib (BTZ) 24 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic skin)
Specification
embryonic skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step
Vector M.O.M Kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 29 2008

Thank you for your enquiry. This product, ab14123 was generated with Recombinant human 14-3-3 sigma protein (26-30kD) as the immunogen. Unfortunately, there is no further epitope information available. I am sorry I can't be of more assistance i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (epithelium)
Specification
epithelium
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Username

Mr. Rudolf Jung

Verified customer

Submitted Feb 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela, Jurkat and SupT1 cell lines)
Loading amount
10 µg
Specification
Hela, Jurkat and SupT1 cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 13 2006

Thank you for your enquiry. Both BSA and glycerol stabilize the antibody. BSA is a carrier protein, and is added to increase the protein concentration of the product, as this helps stabilize the material. Glycerol is a cryoprotectant that prevents...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U251 glial cells; CFBE41o- airway epithelium)
Specification
U251 glial cells; CFBE41o- airway epithelium
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 10 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HCT 116)
Specification
HCT 116
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Sep 12 2005

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up