Recombinant Anti-14-3-3 antibody [EPR5872] (ab133538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5872] to 14-3-3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-14-3-3 antibody [EPR5872]
See all 14-3-3 primary antibodies -
Description
Rabbit monoclonal [EPR5872] to 14-3-3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human 14-3-3 aa 200-300. The exact sequence is proprietary.
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Positive control
- Fetal lung, A549, Human kidney, and Human spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5872 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Entrez Gene: 54401 Mouse
- Entrez Gene: 25578 Rat
- Entrez Gene: 56011 Rat
- Omim: 601288 Human
- Omim: 601289 Human
see all -
Alternative names
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
Images
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All lanes : Anti-14-3-3 antibody [EPR5872] (ab133538) at 1/1000 dilution
Lane 1 : 14-3-3 beta (YWHAB) Human Recombinant Protein 100 ng, with DDDK tagged
Lane 2 : 14-3-3 gamma (YWHAG) Human Recombinant Protein 100 ng, with DDDK tagged
Lane 3 : 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 4 : 14-3-3 zeta (YWHAZ) Human Recombinant Protein 100 ng, with DDDK tagged
Lane 5 : 14-3-3 eta (YWHAH) Human Recombinant Protein 100 ng, with DDDK tagged
Lane 6 : 14-3-3 theta (YWHAQ) Human Recombinant Protein 100 ng, with DDDK tagged
Lane 7 : 14-3-3 sigma (SFN) Human Recombinant Protein 100 ng, with DDDK tagged
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST
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All lanes : Anti-14-3-3 antibody [EPR5872] (ab133538) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : A549 cell lysate
Lane 3 : Human kidney lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133538 has not yet been referenced specifically in any publications.