Overview

  • Product nameAnti-14-3-3 sigma antibody
    See all 14-3-3 sigma primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 sigma
  • Tested applicationsSuitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    SNEEGSEEKGPEV

    , corresponding to internal sequence amino acids 69-81 of Human 14-3-3 sigma

  • Positive control
    • Human breast carcinoma tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab54420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/1000. Heat pre-treatment required.


    Is unsuitable for IHC-Fr or WB.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
      p53-regulated inhibitor of G2/M progression.
    • Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
    • Sequence similaritiesBelongs to the 14-3-3 family.
    • Post-translational
      modifications
      Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
    • Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 3 3 protein sigma antibody
      • 14-3-3 protein sigma antibody
      • 1433S_HUMAN antibody
      • Epithelial cell marker protein 1 antibody
      • Er antibody
      • HME 1 antibody
      • HME1 antibody
      • MGC143283 antibody
      • Mkrn3 antibody
      • Mme1 antibody
      • OTTHUMP00000004242 antibody
      • RP23 137L22.11 antibody
      • SFN antibody
      • SFN protein antibody
      • Stratifin antibody
      • YWHAS antibody
      see all

    Anti-14-3-3 sigma antibody images

    • ab54220, at 1/1000 dilution, staining Human 14-3-3 sigma in breast carcinoma tissue. Paraffin embedded sections. Detection using DAB with methyl green counterstain.

    References for Anti-14-3-3 sigma antibody (ab54420)

    This product has been referenced in:
    • Koo JS & Kim H Hypoxia-related protein expression and its clinicopathologic implication in carcinoma of unknown primary. Tumour Biol 32:893-904 (2011). IHC-P ; Human . Read more (PubMed: 21598042) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab54420.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"