Overview

  • Product nameAnti-14-3-3 sigma antibody
    See all 14-3-3 sigma primary antibodies
  • Description
    Goat polyclonal to 14-3-3 sigma
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow Trout
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma (internal sequence).
    Sequence:

    DLHTLSEDSYKDST


    Database link: P31947

  • Positive control
    • Human skin lysates. Human skin tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:16,000.

ICC/IF Use at an assay dependent concentration. PubMed: 25298405

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody images

  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • Anti-14-3-3 sigma antibody (ab77187) at 0.1 µg/ml + human skin lysate in RIPA buffer at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2.5 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References for Anti-14-3-3 sigma antibody (ab77187)

This product has been referenced in:
  • Plak K  et al. Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Mol Biol Cell 25:4195-204 (2014). ICC/IF ; Dictyostelium discoideum . Read more (PubMed: 25298405) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"