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Synthetic peptide corresponding to Human 14-3-3 sigma (internal sequence).
Our Abpromise guarantee covers the use of ab77187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Antibody detection limit dilution 1:16,000.
|ICC/IF||Use at an assay dependent concentration. PubMed: 25298405|
Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2.5 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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