1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
Detection of 14-3-3 sigma by Western blot of Immunprecipitate.
ab86380, at 1 µg/ml, staining 14-3-3 sigma in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-14-3-3 sigma antibody (ab86380)
has not yet been referenced specifically in any publications.
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