Recombinant
RabMAb

Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667)

Overview

  • Product name
    Anti-14-3-3 sigma antibody [EPR5873(2)]
    See all 14-3-3 sigma primary antibodies
  • Description
    Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 sigma aa 50-150. The exact sequence is proprietary.
    Database link: P31947

  • Positive control
    • Human skin, A431 and HACAT lysates; Human 14-3-3 Sigma recombinant protein; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab193667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • Tissue specificity
    Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • Cellular localization
    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Images

  • All lanes : Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HACAT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 27 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 Sigma recombinant protein with His tag at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 27 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Human full length 14-3-3 sigma recombinant protein is a house-made one with His-Tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab193667 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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