The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 27 kDa).
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Belongs to the 14-3-3 family.
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Western blot - Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667)
Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 Sigma recombinant protein with His tag at 10 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution