Overview

  • Product name
  • Description
    Goat polyclonal to 14-3-3 Tau
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 Tau aa 136-146 (internal sequence).
    Sequence:

    C-DDRKQTIDNSQ


    Database link: NP_006817.1

  • Positive control
    • WB: Human Brain (Hippocampus) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA Use at an assay dependent concentration.

Peptide ELISA: Antibody detection limit dilution 1/4,000. 

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
  • Tissue specificity
    Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    Ser-232 is probably phosphorylated by CK1.
  • Cellular localization
    Cytoplasm. In neurons, axonally transported to the nerve terminals.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein T cell antibody
    • 14 3 3 protein tau antibody
    • 14 3 3 protein theta antibody
    • 14 3 3 tau antibody
    • 14 3 3 theta antibody
    • 14-3-3 protein T-cell antibody
    • 14-3-3 protein tau antibody
    • 14-3-3 protein theta antibody
    • 1433T_HUMAN antibody
    • 1C5 antibody
    • HS1 antibody
    • KCIP1 antibody
    • Protein HS1 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Protein tau antibody
    • tyr3/trp5 monooxygenase activation protein, theta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibody
    • Ywhaq antibody
    see all

Images

  • Anti-14-3-3 Tau antibody (ab77268) at 0.03 µg/ml + Human Brain (Hippocampus) in RIPA buffer at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References

ab77268 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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