Overview

  • Product name
    Anti-14-3-3 theta antibody
  • Description
    Rabbit polyclonal to 14-3-3 theta
  • Host species
    Rabbit
  • Specificity
    ab183075 detects endogenous level of total 14-3-3 theta protein.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human 14-3-3 theta aa 140-154. The exact sequence is proprietary. Genbank: NP_006817
    Sequence:

    QTIDNSQGAYQEAFD


    Database link: P27348

  • Positive control
    • Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/2000. Predicted molecular weight: 28 kDa.
IHC-P 1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : Human A549 cell lysate
    Lane 3 : Human lymphoma tissue lysate
    Lane 4 : mouse NIH 3T3 cell lysate
    Lane 5 : Human colon cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 5 seconds
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)

References

ab183075 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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