Recombinant
RabMAb

Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909)

Overview

  • Product name
    Anti-14-3-3 Theta + Tau antibody [EPR5123]
  • Description
    Rabbit monoclonal [EPR5123] to 14-3-3 Theta + Tau
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 Theta + Tau (N terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, A549 and A431 cell lysates; A549 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • Tissue specificity
      Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
    • Sequence similarities
      Belongs to the 14-3-3 family.
    • Post-translational
      modifications
      Ser-232 is probably phosphorylated by CK1.
    • Cellular localization
      Cytoplasm. In neurons, axonally transported to the nerve terminals.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14-3-3 protein T cell antibody
      • 14-3-3 protein T-cell antibody
      • 14-3-3 protein tau antibody
      • 14-3-3 protein theta antibody
      • 1433T_HUMAN antibody
      • IC5 antibody
      • Protein HS1 antibody
      • Protein tau antibody
      • YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody
      • Ywhaq antibody
      see all

    Images

    • All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • Flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Gao K  et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB ; Human . Read more (PubMed: 25588844) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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