The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for IHC-P or IP.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Tissue specificityAbundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Sequence similaritiesBelongs to the 14-3-3 family.
Post-translational modificationsSer-232 is probably phosphorylated by CK1.
Cellular localizationCytoplasm. In neurons, axonally transported to the nerve terminals.