Overview

  • Product name
  • Description
    Rabbit polyclonal to 14-3-3 zeta
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human 14-3-3 zeta.

  • Positive control
    • WB: Forskolin-treated A431, forskolin-treated NIH 3T3, NIH 3T3, HeLa, HEK293, K562 and Jurkat whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localization
    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Images

  • ICC/IF image of ab125943 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab124431, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-14-3-3 zeta antibody (ab124431) at 1 µg/ml

    Lane 1 : Forskolin-Treated A431 Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Forskolin-Treated NIH 3T3 Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124431 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Mahale S  et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep 6:22 (2016). Read more (PubMed: 28003657) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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