Overview

  • Product name
  • Description
    Rabbit polyclonal to 14-3-3 zeta
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-229 of Human 14-3-3 zeta (UniProt P63104).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt4, Raji and NIH 3T3 cell lysates; HeLaS3 cells; Human Cal27 xenograft tissue,

Properties

Applications

Our Abpromise guarantee covers the use of ab155037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localization
    Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Images

  • Anti-14-3-3 zeta antibody (ab155037) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 28 kDa
  • All lanes : Anti-14-3-3 zeta antibody (ab155037) at 1/2000 dilution

    Lane 1 : Hep G2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLaS3 cells labeling 14-3-3 zeta with ab155037 at 1/100 dilution. Lower panel merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue labeling 14-3-3 zeta with ab155037 at 1/100 dilution.

References

ab155037 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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