Overview

  • Product nameAnti-14-3-3 zeta antibodySee all 14-3-3 zeta primary antibodies ...
  • Description
    Rabbit polyclonal to 14-3-3 zeta
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    AGDDKKGIVDQSQQAY

    , corresponding to amino acids 134 - 149 of Human 14-3-3 zeta

  • Positive control
    • NIH3T3 cells. Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab32622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localizationCytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 delta antibody
    • 14 3 3 protein delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • tyrosine 3 monooxygenase tryptophan 5 monooxygenase activation protein zeta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 zeta antibody images

  • ab32622, at a 1/50 dilution, staining human 14-3-3 zeta in breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue
  • Anti-14-3-3 zeta antibody (ab32622) at 1/25 dilution + lysate prepared from 293 cells

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-14-3-3 zeta antibody (ab32622)

This product has been referenced in:
  • Lubieniecka JM  et al. Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats. PLoS One 6:e19247 (2011). WB ; Rat . Read more (PubMed: 21559420) »
  • Maxwell SA  et al. 14-3-3{zeta} Mediates Resistance of Diffuse Large B Cell Lymphoma to an Anthracycline-based Chemotherapeutic Regimen. J Biol Chem 284:22379-89 (2009). WB ; Human . Read more (PubMed: 19525224) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"