Overview

  • Product name
  • Description
    Rabbit polyclonal to 14-3-3 zeta
  • Specificity
    ab51129 detects endogenous levels of total 14-3-3 zeta protein.
  • Tested applications
    Suitable for: ELISA, WB, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).

  • Positive control
    • Human breast carcinoma tissue or extracts from 293 cells treated with Forskolin.

Properties

Applications

Our Abpromise guarantee covers the use of ab51129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC
ICC/IF
IHC-P
  • Application notes
    ELISA: 1/40000.
    ICC: Use at a concentration of 1 µg/ml.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately ~28 kDa (predicted molecular weight: 28 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • Sequence similarities
      Belongs to the 14-3-3 family.
    • Post-translational
      modifications
      The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
    • Cellular localization
      Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 3 3 delta antibody
      • 14 3 3 protein zeta/delta antibody
      • 14 3 3 protein/cytosolic phospholipase A2 antibody
      • 14 3 3 zeta antibody
      • 14-3-3 protein zeta/delta antibody
      • 1433Z_HUMAN antibody
      • Epididymis luminal protein 4 antibody
      • Epididymis secretory protein Li 3 antibody
      • HEL S 3 antibody
      • HEL4 antibody
      • KCIP-1 antibody
      • KCIP1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • Phospholipase A2 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • YWHAD antibody
      • YWHAZ antibody
      see all

    Anti-14-3-3 zeta antibody images

    • All lanes : Anti-14-3-3 zeta antibody (ab51129)

      Lane 1 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with no peptide
      Lane 2 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with immunising peptide


      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 28 kDa


      Exposure time : 4 minutes
    • This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
    • ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-14-3-3 zeta antibody (ab51129)

    This product has been referenced in:
    • Piersma B  et al. YAP1 Is a Driver of Myofibroblast Differentiation in Normal and Diseased Fibroblasts. Am J Pathol 185:3326-37 (2015). Read more (PubMed: 26458763) »
    • Simeone P  et al. A unique four-hub protein cluster associates to glioblastoma progression. PLoS One 9:e103030 (2014). IHC-P ; Human . Read more (PubMed: 25050814) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast Cancer cell line (MCF7))
    Loading amount
    20 µg
    Specification
    Breast Cancer cell line (MCF7)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 06 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophage)
    Loading amount
    20 µg
    Specification
    macrophage
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Colorectal cancer)
    Loading amount
    30 µg
    Specification
    Colorectal cancer
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Lay Harn Gam

    Verified customer

    Submitted May 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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