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Anti-14-3-3 zeta antibody (ab37560)

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Overview

Product name

Anti-14-3-3 zeta antibody
See all 14-3-3 zeta products (11) ...

Description

Rabbit polyclonal to 14-3-3 zeta

Specificity

This antibody is specific for 14-3-3 zeta + delta.

Tested applications

ELISA, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

A KLH conjugated synthetic peptide selected within aa 200~300 of human 14-3-3 zeta (C-terminal)

Positive control

Jurkat cell lysate and human breast carcinoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Signaling Pathways >> Wnt >> 14-3-3
Signal Transduction >> Adapters >> Cytoplasmic
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

  • Western blot - 14-3-3 zeta + delta antibody (ab37560)Western blot - 14-3-3 zeta + delta antibody (ab37560) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 zeta + delta antibody (ab37560)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 zeta + delta antibody (ab37560) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/1000.

    ELISA: 1/1000.

  • ShowHide1 Image

    WB

     WB: 1/100 - 1/500. Detec...Read more →

    WB: 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sequence similarities

Belongs to the 14-3-3 family.

Post-translational
modifications

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.

Cellular localization

Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.

Target information above from: UniProt accessionP63104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 14 3 3 delta antibody
  • 14 3 3 protein zeta/delta antibody
  • 14 3 3 protein/cytosolic phospholipase A2 antibody
  • 14 3 3 zeta antibody
  • 14-3-3 protein zeta/delta antibody
  • 1433Z_HUMAN antibody
  • KCIP 1 antibody
  • KCIP-1 antibody
  • KCIP1 antibody
  • Phospholipase A2 antibody
  • Protein kinase C inhibitor protein 1 antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
  • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
  • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
  • YWHAD antibody
  • YWHAZ antibody
  • YWHAZ protein antibody
  • Zeta polypeptide antibody
see all

Database links

Anti-14-3-3 zeta antibody images:

  Western blot - 14-3-3 zeta + delta antibody (ab37560)

Western blot - 14-3-3 zeta + delta antibody (ab37560)

Anti-14-3-3 zeta antibody (ab37560) at 1/100 dilution + Jurkat cell lysate

Predicted band size : 28 kDa
Observed band size : 28 kDa
Additional bands at : 15 kDa,60 kDa,90 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 zeta + delta antibody (ab37560)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 zeta + delta antibody (ab37560)

Staining of formalin-fixed and paraffin-embedded human breast cancer tissue with ab37560 at a 1/50 dilution (using a peroxidase-conjugated secondary antibody, followed by AEC staining).

These data demonstrate the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-14-3-3 zeta antibody (ab37560)

ab37560 has not yet been referenced specifically in any publications.

Publishing research using ab37560? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"