Anti-14-3-3 zeta (phospho S58) antibody (ab51109)

Overview

  • Product nameAnti-14-3-3 zeta (phospho S58) antibody
    See all 14-3-3 zeta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 zeta (phospho S58)
  • Specificity14-3-3 zeta (phospho S58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58. The immunogen sequence shows 92% homology with 14-3-3 gamma, eta, and beta/alpha. There may be cross reactivity with these other proteins.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide from Human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-phosphoS-W-R).

  • Positive control
    • NIH/3T3 cell extract; paraffin-embedded human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

Target

  • FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Sequence similaritiesBelongs to the 14-3-3 family.
  • Post-translational
    modifications
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
  • Cellular localizationCytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 delta antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 protein/cytosolic phospholipase A2 antibody
    • 14 3 3 zeta antibody
    • 14-3-3 protein zeta/delta antibody
    • 1433Z_HUMAN antibody
    • Epididymis luminal protein 4 antibody
    • Epididymis secretory protein Li 3 antibody
    • HEL S 3 antibody
    • HEL4 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • MGC111427 antibody
    • MGC126532 antibody
    • MGC138156 antibody
    • Phospholipase A2 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
    • YWHAD antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 zeta (phospho S58) antibody images

  • Ab51109 staining human 14-3-3 zeta in human breast carcinoma tissue in immunohistochemistry using paraffin embedded tissue.
  • All lanes : Anti-14-3-3 zeta (phospho S58) antibody (ab51109) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract (treated with UV for 30 mins) with phosphopeptide immunogen (phospho S58)
    Lane 2 : NIH/3T3 cell extract (treated with UV for 30 mins)


    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ICC/IF image of ab51109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-14-3-3 zeta (phospho S58) antibody (ab51109)

This product has been referenced in:
  • Lachaud C  et al. 14-3-3-Regulated Ca(2+)-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis. Cell Death Differ : (2012). Arabidopsis thaliana . Read more (PubMed: 22935611) »
  • Liu F  et al. Shiga toxin (Stx)1B and Stx2B induce von Willebrand factor secretion from human umbilical vein endothelial cells through different signaling pathways. Blood 118:3392-8 (2011). WB ; Human . Read more (PubMed: 21816831) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Non-reduced Denaturing
Sample Arabidopsis thaliana Purified protein (suspension cells)
Specification suspension cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2013

Thank you for contacting Abcam regarding ab51109. I have confirmed that the immunogen sequence for this antibody is XXX. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Application Western blot
Sample Arabidopsis thaliana Purified protein (Arabidopsis suspension cell culture)
Loading amount 1.5 µg
Specification Arabidopsis suspension cell culture
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: r.t°C
Username

Abcam user community

Verified customer

Submitted Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"