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Abcam’s 14,15 DHET sEH competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate the quantitative measurement in biological samples and cell culture media following proper isolation and purification.
ab175816 is a metabolite formed by the soluble epoxide hydrolase (sEH)-mediated metabolism of 14,15-EET which itself is generated by arachidonic acid epoxygenase activity of cytochromes P450 (CYPs) 2C and 2J. This competitive ELISA kit with an HRP detection system can been used to determine 14,15-DHET levels in biological samples(tissue, plasma, and urine) and cell culture media as a measure of sEH activity. In addition, this assay can also be used to screen for inhibitors of soluble epoxide hydrolase activity. The kit is similar to our 14,15-DHET/EET ELISA kit (DH 2) but also contains the EET substrate. Each kit for a 96-well plate is good for triplicate analyses of up to 12 samples.
|Components||1 x 96 tests|
|14,15-DHET Standard||1 x 2µl|
|14,15-DHET-HRP Conjugates||1 x 12µl|
|14,15-EET||1 x 70µl|
|HRP Buffer||1 x 15ml|
|Pre-coated 96-Well Microplate||1 unit|
|Sample Dilution Buffer||1 x 25ml|
|TMB Substrate||1 x 24ml|
|Wash Buffer Solution||1 x 25ml|
Our Abpromise guarantee covers the use of ab175816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab175816 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"