Overview

  • Product name
    Anti-15 Lipoxygenase 1 antibody
    See all 15 Lipoxygenase 1 primary antibodies
  • Description
    Rabbit polyclonal to 15 Lipoxygenase 1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human 15 Lipoxygenase 1 aa 610-643 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 246

  • Positive control
    • Ramos cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF 1/10 - 1/50.
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA 1/1000.

Target

  • Function
    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway
    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 lipoxygenase 1 antibody
    • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
    • 15 LOX antibody
    • 15 LOX 1 antibody
    • 15-LOX antibody
    • 15LOX 1 antibody
    • ALOX15 antibody
    • Arachidonate 15 lipoxygenase antibody
    • Arachidonate 15-lipoxygenase antibody
    • Arachidonate omega 6 lipoxygenase antibody
    • Arachidonate omega-6 lipoxygenase antibody
    • LOG15 antibody
    • LOX15_HUMAN antibody
    see all

Images

  • All lanes : Anti-15 Lipoxygenase 1 antibody (ab80221)

    Lane 1 : Hela cell line lysate
    Lane 2 : A549 cell line lysate
    Lane 3 : K562 cell line lysate
    Lane 4 : MDA-MB-435 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 73 kDa
  • ab80221 at 1/50 dilution staining 15 Lipoxygenase 1 - C-terminal in human lymph tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ab80221 staining 15 Lipoxygenase 1 in HeLa celss by ICC/IF (Immunocytochemistry/immunofluorescence). An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. Cell nuclear was stained blue with DAPI

  • Anti-15 Lipoxygenase 1 antibody (ab80221) at 1/50 dilution + Ramos cell line lysate at 35 µg

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Katsuyama E  et al. Interleukin-1 receptor-associated kinase-4 (IRAK4) promotes inflammatory osteolysis by activating osteoclasts and inhibiting formation of foreign body giant cells. J Biol Chem 290:716-26 (2015). WB ; Mouse . Read more (PubMed: 25404736) »
  • Latchoumycandane C  et al. Oxidatively truncated phospholipids are required agents of TNFa induced apoptosis. J Biol Chem : (2012). WB ; Human . Read more (PubMed: 22433871) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for submitting an Abreview for ab80221. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested application, we would like to follow up on this to see i...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 06 2012

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(...

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Thank you for your reply.

I am sorry to hear that despite some protocol modifications, this antibody is still not producing the expected result. I would be happy to offer a replacement or credit. We have several other Anti-15 Lipoxygenase 1 ...

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Thank you for your reply with the requested information.

Can I confirm that you are not detecting any bands with the conditions that you have specified? Based on our testing, I would recommend increasing the amount of protein loaded to 60ug ...

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Thank you for contacting Abcam regarding ab80221. I am sorry that you have been experiencing difficulties using this antibody. In order to assist you further and provide some protocol suggestions, can you please specify which application you are using?...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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