Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774)

Overview

  • Product name
    Anti-15 Lipoxygenase 1 antibody [OTI3G8]
    See all 15 Lipoxygenase 1 primary antibodies
  • Description
    Mouse monoclonal [OTI3G8] to 15 Lipoxygenase 1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length, Human 15 Lipoxygenase 1 produced in HEK293T cells (NP_001131).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA; HepG2, HeLa, A549, COS7, MDCK, PC12 and MCF7 cell extracts; Human breast, Human pancreas carcinoma, Human thyroid carcinoma, Human endometrium, Human endometrium adenocarcinoma, Human prostate carcinoma and Human bladder carcinoma; COS7 cells transiently transfected by pCMV6-ENTRY 15 Lipoxygenase 1.
  • General notes

    The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab119774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 75 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • Function
    Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • Pathway
    Lipid metabolism; leukotriene D4 biosynthesis.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 lipoxygenase 1 antibody
    • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
    • 15 LOX antibody
    • 15 LOX 1 antibody
    • 15-LOX antibody
    • 15LOX 1 antibody
    • ALOX15 antibody
    • Arachidonate 15 lipoxygenase antibody
    • Arachidonate 15-lipoxygenase antibody
    • Arachidonate omega 6 lipoxygenase antibody
    • Arachidonate omega-6 lipoxygenase antibody
    • LOG15 antibody
    • LOX15_HUMAN antibody
    see all

Images

  • All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY 15 Lipoxygenase 1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 75 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209418
  • All lanes : Anti-15 Lipoxygenase 1 antibody [OTI3G8] (ab119774) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HeLa cell extracts
    Lane 3 : SVT2 cell extracts
    Lane 4 : A549 cell extracts
    Lane 5 : COS7 cell extracts
    Lane 6 : Jurkat cell extracts
    Lane 7 : MDCK cell extracts
    Lane 8 : PC12 cell extracts
    Lane 9 : MCF7 cell extracts

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 75 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC209486
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human breast tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
  • ab119774 at 1/150 dilution, staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119774 at 1/100, staining 15 Lipoxygenase 1 in COS7 cells transiently transfected with pCMV6-ENTRY 15 Lipoxygenase 1, by immunofluorescence.

References

This product has been referenced in:
  • Li C  et al. Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism. Sci Rep 6:36949 (2016). WB . Read more (PubMed: 27833128) »
  • Shen T  et al. 15-Lipoxygenase and 15-hydroxyeicosatetraenoic acid regulate intravascular thrombosis in pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 309:L449-62 (2015). Read more (PubMed: 26092993) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH6, 100C, 20 min
Permeabilization
No
Specification
prostate cancer
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 05 2015

Thank you very much for sending all of this information.

I'm sending free of charge vials of ab39626 and ab39628 on the order ***, which should arrive on Monday.

Just to confirm, the macrophages were from mice or a different species...

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Thank you so much for contacting us and for letting us know about the trouble with these two antibodies.

We do fully guarantee our products to work as stated on the datasheets in tested species and applications, so if the antibodies did not w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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