Anti-15 Lipoxygenase 2 antibody (ab23691)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab23691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ICC/IF
WB
ICC
  • Application notesICC: Use at a concentration of 6 µg/ml or 1:100
    WB: Use at a concentration of 3 µg/ml or 1:200. Predicted molecular weight: 76 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionConverts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized.
    • Tissue specificityExpressed in hair, prostate, lung and cornea.
    • PathwayLipid metabolism; leukotriene D4 biosynthesis.
    • Sequence similaritiesBelongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 15 LO 2 antibody
      • 15 LOX 2 antibody
      • 15-lipoxygenase 2 antibody
      • 15-LOX-2 antibody
      • 15-LOX-B antibody
      • ALOX15B antibody
      • Arachidonate 15 lipoxygenase second type antibody
      • Arachidonate 15 lipoxygenase type II antibody
      • Arachidonate 15-lipoxygenase B antibody
      • Arachidonate 15-lipoxygenase type II antibody
      • LX15B_HUMAN antibody
      see all

    Anti-15 Lipoxygenase 2 antibody images

    • All lanes : Anti-15 Lipoxygenase 2 antibody (ab23691) at 4 µg/ml

      Lane 1 : Rabbit reticulocyte lysate (30 µg).
      Lane 2 : Human prostate homogenate (30 µg).


      Predicted band size : 76 kDa
      Observed band size : 76 kDa
    • ab23691 staining 15 Lipoxygenase 2 in human artery tissue sections by IHC-Fr (frozen sections). Tissue samples were fixed with acetone and blocked with 0.5% BSA for 10 minutes.  Samples were incubated with primary antibody 1/100  24 hours.  A  biotin-conjugated horse anti Rabbit IgG (H+L) polyclonal  (7 µg/ml) was used as secondary antibody.

      See Abreview

    • Immunofluorescence analysis of murine embryonic fibroblasts, staining 15 Lipoxygenase 2 with ab23691.

      Cells were fixed with paraformaldehyde, permeabilized with 0.2 % Triton-X-100 and blocked with 2% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in 1% BSA in TBST) for 16 hours at 4°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.

      See Abreview

    References for Anti-15 Lipoxygenase 2 antibody (ab23691)

    ab23691 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (murine embryonic fibroblasts)
    Specification murine embryonic fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2 % Triton-X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (artery)
    Specification artery
    Fixative Acetone
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.5%
    Username

    Abcam user community

    Verified customer

    Submitted Nov 24 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2-OS)
    Specification U2-OS
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X-100/PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"