Anti-160 kD Neurofilament Medium antibody (ab9034)
Key features and details
- Rabbit polyclonal to 160 kD Neurofilament Medium
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat, Pig
- Isotype: IgG
Overview
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Product name
Anti-160 kD Neurofilament Medium antibody
See all 160 kD Neurofilament Medium primary antibodies -
Description
Rabbit polyclonal to 160 kD Neurofilament Medium -
Host species
Rabbit -
Specificity
Recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa). -
Tested applications
Suitable for: IHC-FrFl, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Pig
Predicted to work with: Cat, Bird, Reptile -
Immunogen
Recombinant fragment corresponding to Rat 160 kD Neurofilament Medium (C terminal).
Database link: P12839 -
Positive control
- IHC-FrFl: Rat cerebellum WB: Mouse, Rat and Pig brain and spinal cord tissue lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-FrFl |
1/2000.
|
|
WB |
1/5000.
|
Notes |
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IHC-FrFl
1/2000. |
WB
1/5000. |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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Database links
- Entrez Gene: 18040 Mouse
- Entrez Gene: 24588 Rat
- SwissProt: P08553 Mouse
- SwissProt: P12839 Rat
- Unigene: 242832 Mouse
- Unigene: 390700 Mouse
- Unigene: 10971 Rat
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Alternative names
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
Images
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Immunohistochemistry - Free Floating analysis of rat cerebellum section labeling 160 kD Neurofilament Medium with ab9034 at 1/2000 diution (red) and and costained with mouse mAb to GAP43 at 1/2000 diution (green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab9034 strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
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All lanes : Anti-160 kD Neurofilament Medium antibody (ab9034) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Pig spinal cord tissue lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab9034 has been referenced in 16 publications.
- Colombo S et al. Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage. Development 149:N/A (2022). PubMed: 34878101
- Mazzone SB et al. Transcriptional Profiling of Individual Airway Projecting Vagal Sensory Neurons. Mol Neurobiol 57:949-963 (2020). PubMed: 31630330
- Ádám Á et al. Gestational Exposure to Sodium Valproate Disrupts Fasciculation of the Mesotelencephalic Dopaminergic Tract, With a Selective Reduction of Dopaminergic Output From the Ventral Tegmental Area. Front Neuroanat 14:29 (2020). PubMed: 32581730
- Morad T et al. Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis. Polymers (Basel) 12:N/A (2020). PubMed: 33260420
- Landa J et al. Effects of IgLON5 Antibodies on Neuronal Cytoskeleton: A Link between Autoimmunity and Neurodegeneration. Ann Neurol 88:1023-1027 (2020). PubMed: 32740999