Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)

Overview

  • Product nameAnti-160 kD Neurofilament Medium antibody - Neuronal MarkerSee all 160 kD Neurofilament Medium primary antibodies ...
  • Description
    Rabbit polyclonal to 160 kD Neurofilament Medium - Neuronal Marker
  • SpecificityRecognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa).
  • Tested applicationsIHC-FoFr, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
    Predicted to work with: Mouse, Cat, Pig, Bird, Reptiles
  • Immunogen

    Recombinant C-terminal fragment (Rat).

  • Positive control
    • Rat spinal cord

Applications

Our Abpromise guarantee covers the use of ab9034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-Fr
IHC-P
WB
ICC/IF
  • Application notesICC/IF: 1/1000.
    IHC-F: 1/1000.
    IHC-P: 1/1000.
    IHC-Fr: 1/1000.
    WB: 1/5000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
    • Cellular localizationCytoplasm
    • Database links
    • Alternative names
      • 150kDa medium antibody
      • 160 kDa neurofilament protein antibody
      • NEF 3 antibody
      • NEF3 antibody
      • NEFM antibody
      • Neurofilament 3 antibody
      • Neurofilament medium polypeptide antibody
      • Neurofilament protein medium antibody
      • Neurofilament triplet M protein antibody
      • Neurofilament3 antibody
      • NF M antibody
      • NF160 antibody
      • NFM antibody
      see all

    Anti-160 kD Neurofilament Medium antibody - Neuronal Marker images

    • The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
    • Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/20000 dilution + whole rat cerebellum homogenate
    • ICC/IF image of ab9034 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9034, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution

      Lane 1 : Cow 160 kD Neurofilament Medium full length protein (ab73759) at 0.1 µg
      Lane 2 : Cow 160 kD Neurofilament Medium full length protein (ab73759) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)

    This product has been referenced in:
    • Stegeman S  et al. Loss of Usp9x disrupts cortical architecture, hippocampal development and TGFß-mediated axonogenesis. PLoS One 8:e68287 (2013). Read more (PubMed: 23861879) »
    • Deng Y  et al. Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and phosphorylation of tau and neurofilaments in the brain: Implication for Alzheimer's disease. Am J Pathol 175:2089-98 (2009). WB, IHC-P ; Rat . Read more (PubMed: 19815707) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain tissue)
    Specification Brain tissue
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 12 2011

    We have just received the following information from Novus: "I would guess 10 mg/ml based upon feedback on previous rabbits." I hope this information helps, best of luck with your stainings,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"