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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab30309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent concentration. PubMed: 20635090
ICC/IF ICC/IF: 1/100 - 1/1000.
WB WB: 1/1000 - 1/5000. Predicted molecular weight: 102 kDa.

Target

  • RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localizationCytoplasm
  • Database links
    • Alternative names
        150kDa medium antibody160 kDa neurofilament protein antibodyNEF 3 antibody
        NEF3 antibodyNEFM antibodyNeurofilament 3 antibodyNeurofilament medium polypeptide antibodyNeurofilament protein medium antibodyNeurofilament triplet M protein antibodyNeurofilament3 antibodyNF M antibodyNF160 antibodyNFM antibody
      see all

    References for Anti-160 kD Neurofilament Medium antibody (ab30309)

    This product has been referenced in:
    • Seidel K  et al. Axonal inclusions in spinocerebellar ataxia type 3. Acta Neuropathol 120:449-60 (2010). IHC-P ; Human . Read more (PubMed: 20635090) »
    • Zhao X  et al. Neuronal PPARgamma deficiency increases susceptibility to brain damage after cerebral ischemia. J Neurosci 29:6186-95 (2009). WB ; Mouse . Read more (PubMed: 19439596) »

    See all 2 Publications for this product

    Publishing research using ab30309 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-160 kD Neurofilament Medium antibody

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (Human Brain)
    Loading amount 15 µg
    Specification Human Brain
    Gel Running Conditions Reduced Denaturing (12%T)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Feb 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"