Recombinant Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y]
See all 160 kD Neurofilament Medium primary antibodies -
Description
Rabbit monoclonal [EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y cell lysates; human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR580(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P |
Notes |
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WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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Database links
- Entrez Gene: 4741 Human
- Omim: 162250 Human
- SwissProt: P07197 Human
- Unigene: 458657 Human
- Unigene: 730072 Human
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Alternative names
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution
Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
Lane 2 : Untreated SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab68142 has not yet been referenced specifically in any publications.