Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)

Overview

  • Product nameAnti-160 kD Neurofilament Medium antibody [3H11]
    See all 160 kD Neurofilament Medium primary antibodies
  • Description
    Mouse monoclonal [3H11] to 160 kD Neurofilament Medium
  • SpecificitySpecifically recognizes the medium neurofilament subunit (~145-170 kDa).
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Human, a wide range of other species
  • Immunogen

    A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.

Properties

Applications

Our Abpromise guarantee covers the use of ab7256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/2500.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/500.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localizationCytoplasm
  • Database links
  • Alternative names
    • 150kDa medium antibody
    • NEF3 antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • NF160 antibody
    • NFM antibody
    see all

Anti-160 kD Neurofilament Medium antibody [3H11] images

  • Lane 1 : Coomassie Blue stain
    Lane 2 : Heavy neurofilament antibody
    Lane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)
    Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)
    Lane 5 : alpha Internexin antibody
    Lane 6 : GFAP antibody

    Lane 1 : Cytoskeletal homogenates of rat spinal cord
    Lane 2 : Cytoskeletal homogenates of rat spinal cord
    Lane 3 : Cytoskeletal homogenates of rat spinal cord
    Lane 4 : Cytoskeletal homogenates of rat spinal cord
    Lane 5 : Cytoskeletal homogenates of rat spinal cord
    Lane 6 : Cytoskeletal homogenates of rat spinal cord

References for Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)

This product has been referenced in:
  • Zhang L  et al. {gamma}-Diketone Axonopathy: Analyses of Cytoskeletal Motors and Highways in CNS Myelinated Axons. Toxicol Sci 117:180-9 (2010). WB ; Rat . Read more (PubMed: 20554699) »

See 1 Publication for this product

Product Wall

This antibody would probably detect NF in chicken using IHC. However, since it has not been tested, we can't guarantee results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"