Anti-160 kD Neurofilament Medium antibody (ab72998)
Key features and details
- Chicken polyclonal to 160 kD Neurofilament Medium
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgY
Overview
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Product name
Anti-160 kD Neurofilament Medium antibody
See all 160 kD Neurofilament Medium primary antibodies -
Description
Chicken polyclonal to 160 kD Neurofilament Medium -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat 160 kD Neurofilament Medium protein expressed in and purified from E. coli.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 145 kDa (predicted molecular weight: 96 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 145 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
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Database links
- Entrez Gene: 24588 Rat
- SwissProt: P12839 Rat
- Unigene: 10971 Rat
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Alternative names
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
Images
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Anti-160 kD Neurofilament Medium antibody (ab72998) at 1/5000 dilution + rat cerebral cortex lysate
Predicted band size: 96 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab72998 has been referenced in 2 publications.
- Decourtye L et al. Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation. PLoS One 13:e0193196 (2018). PubMed: 29466413
- Decourtye L et al. IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice. PLoS One 12:e0170083 (2017). PubMed: 28076448