Anti-160 kD Neurofilament Medium antibody [NF-09] (ab7794)

Overview

  • Product name
    Anti-160 kD Neurofilament Medium antibody [NF-09]
    See all 160 kD Neurofilament Medium primary antibodies
  • Description
    Mouse monoclonal [NF-09] to 160 kD Neurofilament Medium
  • Specificity
    This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, ICC, IHC-P, WB, IHC (PFA fixed), ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Cat, Human, Pig
    Predicted to work with: a wide range of other species, Mammal
  • Immunogen

    Pellet of pig brain cold stable proteins after depolymerization of microtubules.

Properties

Applications

Our Abpromise guarantee covers the use of ab7794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000.
IHC (PFA fixed) 1/500.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localization
    Cytoplasm
  • Database links
  • Alternative names
    • 150kDa medium antibody
    • NEF3 antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • NF160 antibody
    • NFM antibody
    see all

Anti-160 kD Neurofilament Medium antibody [NF-09] images

  • ab7794 staining 160 kD Neurofilament Medium (red) in Human nerve surrounding common hepatic artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in Da Vinci Green diluent) for 16 hours at 4°C. An undiluted HRP-conjugated mouse polymer was used as the secondary antibody. Tyrosin Hydroxylase was stainded green with ab112

    See Abreview

  • Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

  • Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794.

    Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.
  • ab7794 at a dilution of 1/1000, staining 160 kD Neurofilament Medium (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.
  • ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

References for Anti-160 kD Neurofilament Medium antibody [NF-09] (ab7794)

This product has been referenced in:
  • Bragança B  et al. Ion Fluxes through KCa2 (SK) and Cav1 (L-type) Channels Contribute to Chronoselectivity of Adenosine A1 Receptor-Mediated Actions in Spontaneously Beating Rat Atria. Front Pharmacol 7:45 (2016). Read more (PubMed: 27014060) »
  • Marignier R  et al. Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid. J Neuroinflammation 13:111 (2016). Read more (PubMed: 27193196) »

See all 14 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Nerve surrounding common hepatic artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Nerve surrounding common hepatic artery
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (nerve)
Specification
nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2010

The originator of ab7794 uses cell brain lysate (pig or mouse) as a positive control in Western blots. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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