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Pellet of pig brain cold stable proteins after depolymerization of microtubules.
Our Abpromise guarantee covers the use of ab7794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC (PFA fixed)||1/500.|
|ICC/IF||Use at an assay dependent concentration.|
ab7794 staining 160 kD Neurofilament Medium (red) in Human nerve surrounding common hepatic artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in Da Vinci Green diluent) for 16 hours at 4°C. An undiluted HRP-conjugated mouse polymer was used as the secondary antibody. Tyrosin Hydroxylase was stainded green with ab112
Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.
ab7794 at a dilution of 1/1000, staining 160 kD Neurofilament Medium (Alexa 488 secondary at 1/2000) on rat brain tissue (30
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).