Anti-17 beta Estradiol 3 antibody (ab50660)

Overview

  • Product nameAnti-17 beta Estradiol 3 antibody
  • Description
    Goat polyclonal to 17 beta Estradiol 3
  • SpecificityCross reactivities with related molecules; Estrone < 1%, Estriol < 1%, Testosterone < 1%, DHEA < 1%, Androstenedione 0%.
  • Tested applicationsSuitable for: RIA, ELISAmore details
  • Immunogen

    17ß Estradiol-3 conjugated to BSA

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA
ELISA
  • Application notesEIA: Titre 1/1100000.
    RIA: Titre 1/1100000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance17 beta estradiol is an estrogenic hormone, C18H24O2, produced by the ovaries and used in treating estrogen deficiency and breast cancer. Estradiol, like other sex steroids, is derived from cholesterol. After side chain cleavage and either utilizing the delta-5 pathway or the delta-4 pathway androstenedione is the key intermediary. Androstendione is either converted to testosterone which in turn undergoes aromatization to estradiol, or, alternatively, androstendione is aromatized to estrone which is converted to estradiol. During the reproductive years most estradiol in women is produced by the granulosa cells of the ovaries by aromatization of testosterone from the theca cells, or conversion of estrone to estradiol. Smaller amounts of estradiol are also produced by the adrenal cortex. In men, the testes produce some estradiol. An additional source of estradiol in both sexes is peripheral aromatization of testosterone to estradiol. Particularly fat cells are active to convert precursors to estradiol, and will continue to do so even after the menopause. Estradiol enters cells freely and interacts with a target cell receptor. When the estrogen receptor has bound its ligand it can enter the nucleus of the target cell, and regulate gene transcription which leads to formation of messenger RNA. The mRNA interacts with ribosomes to produce specific proteins that express the effect of estradiol upon the target cell.
    • Cellular localizationSecreted

    References for Anti-17 beta Estradiol 3 antibody (ab50660)

    ab50660 has not yet been referenced specifically in any publications.

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    We guarantee ab50660 to work in RIA, Enzyme Immunoassays. If you wish to test the antibody in another application apart from these, then you would qualify for our testing discount program. The link below provides mo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"