Anti-17 beta Estradiol 6 antibody [7G4] (ab23449)

Overview

  • Product nameAnti-17 beta Estradiol 6 antibody [7G4]
  • Description
    Mouse monoclonal [7G4] to 17 beta Estradiol 6
  • Specificityab23449 reacts with estradiol-17beta. No crossreactivity is seen with estrone, esrriol, estrone sulphate, 16-ketoestradiol, 2-hydroxyestradiol, progesterone, androstenedione, testosterone, or cortisol when measuring on tritium-labeled steroids in radioimmunoassay.
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Estradiol-17b-6-CMO coupled to BSA

  • EpitopeEpitope specific for the hormone, no reactivity to the CMO linker
  • General notes


    HYB 057-02 reacts specifically with estradiol-17beta with a measured Ka of 3x10E9 in a radioimmunoassay. HYB 057-02 can be cleaved with papain and reduced to generate Fab fragments with a Ka of 13.4x10E9

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesHYB 057-02 reacts specifically with estradiol-17beta with a measured Ka of 3x10E9 in a radioimmunoassay. HYB 057-02 can be cleaved with papain and reduced to generate Fab fragments with a Ka of 13.4x10E9
  • ClonalityMonoclonal
  • Clone number7G4
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1500.

Target

  • RelevanceEstradiol is the most potent estrogen of a group of endogenous estrogen steroids which includes estrone and estriol. In women estradiol is responsible for growth of the breast and reproductive epithelia, maturation of long bones and development of the secondary sexual characteristics. Estradiol is produced mainly by the ovaries with secondary production by the adrenal glands and conversion of steroid precursors into estrogens in fat tissue.
  • Cellular localizationType I Membrane
  • Alternative names
    • Estradiol 17 beta antibody

References for Anti-17 beta Estradiol 6 antibody [7G4] (ab23449)

This product has been referenced in:
  • Pajunen M  et al. Cloning, sequencing, expression and characterization of three anti-estradiol-17beta Fab fragments. Biochim Biophys Acta 1351:192-202 (1997). Read more (PubMed: 9116033) »
  • Lamminmäki U  et al. Structural analysis of an anti-estradiol antibody. Mol Immunol 34:1215-26 (1997). Read more (PubMed: 9566768) »

See all 2 Publications for this product

Product Wall

Thank you for your patience in this matter. The originator of ab23449 (17 beta Estradiol 6 antibody [HYB 057-02]) has informed us that the product has been processed to prevent reaction with BSA, so there is only reaction with beta estradiol. I w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"