Overview

  • Product name
    Anti-17 beta Estradiol antibody [7G4]
    See all 17 beta Estradiol primary antibodies
  • Description
    Mouse monoclonal [7G4] to 17 beta Estradiol
  • Specificity
    ab23449 reacts with estradiol-17beta. No crossreactivity is seen with estrone, esrriol, estrone sulphate, 16-ketoestradiol, 2-hydroxyestradiol, progesterone, androstenedione, testosterone, or cortisol when measuring on tritium-labeled steroids in radioimmunoassay.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Estradiol-17b-6-CMO coupled to BSA

  • Epitope
    Epitope specific for the hormone, no reactivity to the CMO linker
  • General notes


    HYB 057-02 reacts specifically with estradiol-17beta with a measured Ka of 3x10E9 in a radioimmunoassay. HYB 057-02 can be cleaved with papain and reduced to generate Fab fragments with a Ka of 13.4x10E9

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    HYB 057-02 reacts specifically with estradiol-17beta with a measured Ka of 3x10E9 in a radioimmunoassay. HYB 057-02 can be cleaved with papain and reduced to generate Fab fragments with a Ka of 13.4x10E9
  • Clonality
    Monoclonal
  • Clone number
    7G4
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1500.

Target

  • Relevance
    17 beta Estradiol is a steriod hormone that is produced in the ovary, placenta, testis, and possibly the adrenal cortex.
  • Cellular localization
    Secreted
  • Alternative names
    • 17 beta Oestradiol antibody
    • estradiol antibody

References for Anti-17 beta Estradiol antibody [7G4] (ab23449)

This product has been referenced in:
  • Pajunen M  et al. Cloning, sequencing, expression and characterization of three anti-estradiol-17beta Fab fragments. Biochim Biophys Acta 1351:192-202 (1997). Read more (PubMed: 9116033) »
  • Lamminmäki U  et al. Structural analysis of an anti-estradiol antibody. Mol Immunol 34:1215-26 (1997). Read more (PubMed: 9566768) »

See all 2 Publications for this product

Product Wall

Thank you for your patience in this matter. The originator of ab23449 (17 beta Estradiol 6 antibody [HYB 057-02]) has informed us that the product has been processed to prevent reaction with BSA, so there is only reaction with beta estradiol. I w...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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