Anti-17 beta Estradiol antibody [ESTR-1] (ab20626)

Overview

  • Product name
    Anti-17 beta Estradiol antibody [ESTR-1]
    See all 17 beta Estradiol primary antibodies
  • Description
    Mouse monoclonal [ESTR-1] to 17 beta Estradiol
  • Specificity
    Reacts with 17 beta Estradiol BSA conjugate and free Estradiol. No cross reactivity with BSA.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    17 beta Estradiol conjugated to BSA.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab54122 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab20626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Can be used in competitive assay of 17 beta Estradiol.
    Not tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.Use at an assay dependent dilution.

  • Target

    • Relevance
      17 beta Estradiol is a steriod hormone that is produced in the ovary, placenta, testis, and possibly the adrenal cortex.
    • Cellular localization
      Secreted
    • Alternative names
      • 17 beta Oestradiol antibody
      • estradiol antibody

    References

    This product has been referenced in:
    • von Schalburg KR  et al. Subcellular localization and characterization of estrogenic pathway regulators and mediators in Atlantic salmon spermatozoal cells. Histochem Cell Biol N/A:N/A (2017). Read more (PubMed: 28983690) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
    Sample
    Salmo salar Cell (testicular)
    Specification
    testicular
    Permeabilization
    Yes - methyl methacrylate/Butyl methacrylate
    Fixative
    glutaraldehyde-formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 25 2015

    Thank you for your enquiry. The isolelectric points and the percentage of glycosylation have not, to our knowledge,been determined for the three antibodies you asked about (ab35799, ab20626, and, in a separate e-mail, ab20877). These are all IgG1 isoty...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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