For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|17 OH Progesterone Control||1 x 1ml|
|17 OH Progesterone Standard 0 – 0 ng/mL||1 x 1ml|
|17 OH Progesterone Standard 1 – 0.2 ng/mL||1 x 1ml|
|17 OH Progesterone Standard 2 – 0.6 ng/mL||1 x 1ml|
|17 OH Progesterone Standard 3 – 2.0 ng/mL||1 x 1ml|
|17 OH Progesterone Standard 4 – 6.0 ng/mL||1 x 1ml|
|17 OH Progesterone Standard 5 – 20.0 ng/mL||1 x 1ml|
|17 OH Progesterone-HRP Conjugate||1 x 22ml|
|Anti-17 OH Progesterone IgG Coated Microplate (12 x 8 wells)||1 unit|
|Cover foils||1 unit|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108668 has not yet been referenced specifically in any publications.