25x TBS (pH 7.4) (ab64248)
Overview
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Product name
25x TBS (pH 7.4)
See all TBS reagents -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Tris Buffered Saline (TBS) wash buffer ab64248 for use during immunolabeling techniques such as IHC.
In order to provide this product at 25x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution:
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 7
Constituents: 22% Sodium chloride, 20.8% Tris HCl
Buffer 25x concentrated. Made from Tris base and HCl. -
Concentration information loading...
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Research areas
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Relevance
TBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies. -
Alternative names
- Tris buffered saline
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent dilution.
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Notes |
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IHC-P
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab64248 has not yet been referenced specifically in any publications.