Anti-2 Hydroxy phytanoyl CoA lyase antibody (ab24423)

Overview

  • Product nameAnti-2 Hydroxy phytanoyl CoA lyase antibody
    See all 2 Hydroxy phytanoyl CoA lyase primary antibodies
  • Description
    Mouse polyclonal to 2 Hydroxy phytanoyl CoA lyase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Fusion protein:

    VTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNY YTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMG

    , corresponding to amino acids 327/426 of Human 2 Hydroxy phytanoyl CoA lyase

  • General notesProduced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab24423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

Anti-2 Hydroxy phytanoyl CoA lyase antibody images

  • All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody (ab24423) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 64 kDa

References for Anti-2 Hydroxy phytanoyl CoA lyase antibody (ab24423)

ab24423 has not yet been referenced specifically in any publications.

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