You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-200 kD Neurofilament Heavy antibody [3G3] (ab19386)

Submit an Abreview Submit a Question

Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [3G3]See all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Mouse monoclonal [3G3] to 200 kD Neurofilament Heavy
  • Tested applicationsIHC-P, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Immunogen corresponds to 226 amino acids of the C terminus of rat neurofilament, heavy chain.

  • Positive controlRat brain microsome

Properties

Applications

Our Abpromise guarantee covers the use of ab19386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ICC/IF
WB
  • Application notesIF: Use at a concentration of 5 µg/ml.
    IHC-P: Use at a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 112 kDa). Most sources give a predicted MW of ~100 kDa. MA1-2012 recognises neurofilament triplet protein P200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

      • Target information above from: UniProt accession P12036 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        200 kDa neurofilament protein antibodyKIAA0845 antibodyNEFH antibody
        Neurofilament H antibodyNeurofilament heavy polypeptide 200kD antibodyNeurofilament heavy polypeptide 200kDa antibodyNeurofilament heavy polypeptide antibodyNeurofilament triplet H protein antibodyNF H antibodyNF-H antibodyNFH antibodyNFH_HUMAN antibody
      see all

    Anti-200 kD Neurofilament Heavy antibody [3G3] images

    • Immunocytochemistry/ Immunofluorescence - 200 kD Neurofilament Heavy antibody [3G3] (ab19386)
      Immunocytochemistry/ Immunofluorescence - 200 kD Neurofilament Heavy antibody [3G3] (ab19386)
      Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.

    References for Anti-200 kD Neurofilament Heavy antibody [3G3] (ab19386)

    ab19386 has not yet been referenced specifically in any publications.

    Publishing research using ab19386 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab19386 Submit a Question for ab19386

    There are currently no Abreviews or Questions for ab19386.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"