Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158)

Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [AH1]See all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Mouse monoclonal [AH1] to 200 kD Neurofilament Heavy
  • Specificityab78158 recognizes phosphorylated 200 kD Neurofilament Heavy KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. ab78158 recognizes 200 kD Neurofilament Heavy strongly in all mammals tested to date
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Chicken, Cow
    Predicted to work with: all Mammals
  • Immunogen

    Native 200 kD Neurofilament Heavy purified from bovine spinal cord.

  • Positive control
    • Crude rat spinal cord extract. Mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab78158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC/IF: 1/1000.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/10000 using chemiluminescence. Predicted molecular weight: 112 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications
      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt
    • Database links
    • Alternative names
      • 200 kDa neurofilament protein antibody
      • NEFH antibody
      • Neurofilament H antibody
      • Neurofilament heavy polypeptide 200 kD antibody
      • Neurofilament heavy polypeptide 200 kDa antibody
      • Neurofilament heavy polypeptide 200kD antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF 200 antibody
      • NF H antibody
      • NF-H antibody
      • NF200 antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    Anti-200 kD Neurofilament Heavy antibody [AH1] images

    • Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158) at 1/10000 dilution + Crude rat spinal cord extract
      developed using the ECL technique

      Predicted band size : 112 kDa
    • Mixed neuron/glial cultures stained with ab78158 (green), at 1/1000 dilution, and also stained with a rabbit polyclonal antibody to neurofilament NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while ab78158 binds to only heavily phosphorylated forms of 200 kD Neurofilament Heavy which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated 200 kD Neurofilament Heavy they appear golden in color. Blue shows the distribution of DNA.

    References for Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158)

    ab78158 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"