Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158)

Overview

• Product nameAnti-200 kD Neurofilament Heavy antibody [AH1]See all 200 kD Neurofilament Heavy primary antibodies ...
• Description
  Mouse monoclonal [AH1] to 200 kD Neurofilament Heavy
• Specificityab78158 recognizes phosphorylated 200 kD Neurofilament Heavy KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. ab78158 recognizes 200 kD Neurofilament Heavy strongly in all mammals tested to date
• Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Chicken, Cow
  Predicted to work with: all Mammals
• Immunogen

  Native 200 kD Neurofilament Heavy purified from bovine spinal cord.

• Positive controlCrude rat spinal cord extract. Mixed neuron/glial cultures.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberAH1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class IV
  • Neurofilaments

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Axon marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
• Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
  Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
  Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

Target information above from: UniProt accession P12036 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

• Alternative names
  200 kDa neurofilament protein antibodyNEFH antibodyNeurofilament H antibody

  Neurofilament heavy polypeptide 200 kD antibodyNeurofilament heavy polypeptide 200 kDa antibodyNeurofilament heavy polypeptide 200kD antibodyNeurofilament heavy polypeptide 200kDa antibodyNeurofilament heavy polypeptide antibodyNeurofilament triplet H protein antibodyNF 200 antibodyNF H antibodyNF-H antibodyNF200 antibodyNFH antibodyNFH_HUMAN antibody

  see all

Anti-200 kD Neurofilament Heavy antibody [AH1] images

• 

  Western blot - 200 kD Neurofilament Heavy antibody [AH1] (ab78158)
  Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158) at 1/10000 dilution + Crude rat spinal cord extract
  developed using the ECL technique
  
  Predicted band size : 112 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - 200 kD Neurofilament Heavy antibody [AH1] (ab78158)
  Mixed neuron/glial cultures stained with ab78158 (green), at 1/1000 dilution, and also stained with a rabbit polyclonal antibody to neurofilament NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while ab78158 binds to only heavily phosphorylated forms of 200 kD Neurofilament Heavy which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated 200 kD Neurofilament Heavy they appear golden in color. Blue shows the distribution of DNA.