Anti-200 kD Neurofilament Heavy antibody [NAP4] (ab32660)

Overview

• Product nameAnti-200 kD Neurofilament Heavy antibody [NAP4]See all 200 kD Neurofilament Heavy primary antibodies ...
• Description
  Mouse monoclonal [NAP4] to 200 kD Neurofilament Heavy
• SpecificityThis antibody recognizes phosphorylated NFH sequence n-KSP-c (lysine serine proline). Some cross reactivity to the phosphorylated n-KSP-c of subunit unit NFM may be observed in some species.
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Chicken
  Predicted to work with: all Mammals
• Immunogen

  Full length native protein (purified) from Pig spinal cord.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate).
  Constituents: 50% Glycerol, 1mg/ml BSA.
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberNAP4
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class IV
  • Neurofilaments

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Axon marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

Applications

Target

• FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
• Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
  Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
  Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

Target information above from: UniProt accession P12036 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

• Alternative names
  200 kDa neurofilament protein antibodyKIAA0845 antibodyNEFH antibody

  Neurofilament H antibodyNeurofilament heavy polypeptide 200kD antibodyNeurofilament heavy polypeptide 200kDa antibodyNeurofilament heavy polypeptide antibodyNeurofilament triplet H protein antibodyNF H antibodyNF-H antibodyNFH antibodyNFH_HUMAN antibody

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